Composite

Part:BBa_K1487027

Designed by: Samantha Bermudez   Group: iGEM14_UT-Dallas   (2014-10-03)


Promoter + Guide RNA (gRNA) target for tcpA

This part contains guide RNA (gRNA) that can target toxin coregulated pilus A (tcp) gene under strong constitutive promoter J23119.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None